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Lipids Total cholesterol X X X X HDL-cholesterol X X X X LDL-cholesterol X X X X Triglycerides X X X X Apolipoprotein A1 X Apolipoprotein B X X X Adiponectin X Iron status Ferritin X Iron X Total iron-binding capacity X Transferrin saturation c X Hepcidin X d Haemoglobin X Haematocrit, mean corpuscular ahemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) X Metabolites Homocysteine, cysteine, methionine, serine, glycine, cystathionine, tryptophan, kynurenine, folate, cobalamin, pyridoxal phosphate, pyridoxal, pyridoxic acid, pyridoxamine, pyridoxine, riboflavin, neopterine, cotinine, paba-glutamine, methylmalonic aciduria type A protein X Thyroid function Thyroid-stimulating hormone X X Free T4 X X Anti-TPO X X Liver Alanine aminotransferase X Bilirubin X Inflammation C-reactive protein X X X High sensitive C-reactive protein X X Macrophage migration inhibitory factor (MIF) X d IL-18 X Macrophage colony-stimulating factor (M-CSF) X IL-1?, IL-6, IL-8, VEGF X Erythrocytes, leucocytes X X Thrombocytes, neutrophils, lymphocytes, monocytes, eosinophils, basophils X Nitrite, protein, glucose, ketones, urobilinogen, bilirubin, epithelial cells, bacteria, casts X Renal function Creatinine X X X X Albumin X X X Urea X X Diabetes Fasting glucose X X X Insulin X Iodine status Iodine X. NBS-1 a ( N = 6468). NBS-2-NIMA1 b blood samples ( N = 1517). NBS-2-NIMA2 b blood samples ( N = 95). NBS-2-NIMA2 urine samples ( N = 1066). NBS-2-NIMA3 b blood samples (N=302). Lipids Total cholesterol X X X X HDL-cholesterol X X X X LDL-cholesterol X X X X Triglycerides X X X X Apolipoprotein A1 X Apolipoprotein B X X X Adiponectin X Iron status Ferritin X Iron X Total iron-binding capacity X Transferrin saturation c X Hepcidin X d Haemoglobin X Haematocrit, mean corpuscular ahemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) X Metabolites Homocysteine, cysteine, methionine, serine, glycine, cystathionine, tryptophan, kynurenine, folate, cobalamin, pyridoxal phosphate, pyridoxal, pyridoxic acid, pyridoxamine, pyridoxine, riboflavin, neopterine, cotinine, paba-glutamine, methylmalonic aciduria type A protein X Thyroid function Thyroid-stimulating hormone X X Free T4 X X Anti-TPO X X Liver Alanine aminotransferase X Bilirubin X Inflammation C-reactive protein X X X High sensitive C-reactive protein X X Macrophage migration inhibitory factor (MIF) X d IL-18 X Macrophage colony-stimulating factor (M-CSF) X IL-1?, IL-6, IL-8, VEGF X Erythrocytes, leucocytes X X Thrombocytes, neutrophils, lymphocytes, monocytes, eosinophils, basophils X Nitrite, protein, glucose, ketones, urobilinogen, bilirubin, epithelial cells, bacteria, casts X Renal function Creatinine X X X X Albumin X X X Urea X X Diabetes Fasting glucose X X X Insulin X Iodine status Iodine X. The first phase of NBS (NBS-1) has been followed by four additional phases (NBS-2, -3, -4 and -5) across the period 2002-16. NBS-NIMA1, -2 and -3 are sub-studies of NBS-2, focused on cardiovascular risk prediction using non-invasive measurements of atherosclerosis (NIMA). Samples were non-fasting and taken during the day. Time of blood sampling was recorded. Fasting samples taken in the morning. Transferrin saturation is calculated by dividing serum iron by total iron-binding capacity. Hepcidin and macrophage migration inhibitory factor have been measured in 2998 samples. Initially, genome-wide genotyping was carried out for 1980 samples using the Illumina HumanHapCNV370-Duo BeadChip platform, of which 1819 survived quality control (QC). Currently, we have genome-wide genotype data measured with the Illumina HumanOmniExpress-12 and -24 BeadChip available for 5363 samples; 5292 of these passed a call rate threshold of 95% and were imputed using the 1000 Genomes phase1 v3 14 together with Genome of The Netherlands (GoNL) release 5 15 data as reference. Pre-imputation QC on the marker level consisted of a minor allele frequency (MAF) > 0. Imputation was performed using the Impute2 pipeline developed by the GoNL team; see [http://www. Post-imputation quality control consisted of exclusion of population outliers using principal component analysis, exclusion of sex discrepancies based on a comparison of genotype data and clinical data, and a relatedness check, which resulted finally in 4745 samples available for genome-wide analyses. In addition, 1931 NBS samples have been chipped with the Illumina HumanExome BeadChip, which covers putative functional exonic variants and is focused on the measurement of rare (MAF 17 After quality control, that is sample call rate > 95%, removal of sex discrepancies, a relatedness check, a heterozygosity check, exclusion of population outliers, marker call rate > 98%, exclusion of markers that should be treated with caution’see Exome Chip Design Wiki: [http://genome.
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