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Second, the Northern evaluation (Fig. 3) shows that the precursor mRNA is present exclusively in the cerebral ganglia, where the F cluster is located. Third, immunocytochemistry directed against the 1714 Da peptide sequence stains neurons within the F cluster of the cerebral ganglia and the UL and AT nerves (Fig. 4), best steroid for testosterone replacement therapy4. Furthermore, the immunostained neurons in the F cluster have an identical morphology and distribution as the CFt class of neurons, which had been the premise for the purification of the peptide (Rubakhin et al, best steroid for testosterone replacement therapy5., 1999), best steroid for testosterone replacement therapy5. Fourth, further peptides predicted by the precursor may be seen within the MALDI-MS spectra from these neurons. These peaks have been subsequently used to outline the processing of the precursor, best steroid for testosterone replacement therapy6. Semi-nested degenerate PCR yielded a single clone which, upstream of the degenerate primer, encoded the expected upstream amino acid sequence.
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